Bpsbioscience/BRD2 (BD1), His-tag/31022/100 µg

来自 : 发布时间：2025-01-16

Species: Human Host Species: E. coli MW: 15.5 kDa Genbank #: NM_005104 Tag(s): N-terminal His-tag a.a: 65-187 Description:Human Bromodomain containing 2, also known as BRD2, RING3 or RNF3, GenBank Accession No. NM_005104, a.a. 65 – 187 corresponding to bromodomain 1 with N-terminal His-tag, MW = 15.5 kDa, expressed in an E. coli expression system. UniProt P25440 Synonym(s): BRD2, RING, RNF3, Bromodomain containing 2 Assay Conditions:

BRD2 is incubated with biotinylated acetyl-histone peptide substrate (BPS #33000) in BRD assay buffer (BPS #33001) in a 10 µl reaction for 1 hour at RT. AlphaLISA^® ® Nickel Chelate acceptor beads (PerkinElmer) are added, followed by Alphascreen^®® Streptavidin-conjugated donor beads (PerkinElmer). After 1 hour minute incubation at RT, Alphacounts are measured.

Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, and 10% glycerol. Format: Aqueous buffer solution Storage / Stability: >6 months at –80°C. Application(s): Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling. Reference(s): 1. Umehara, T., et al., FEBS Lett. 2010 Sep 24; 584(18): 3901-3908.2. Shang, E., et al., J Cell Biochem. 2011 Oct; 112(10): 2784-2793. Warning(s): Avoid freeze/thaw cycles. Scientific Category: Bromodomain Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.